{"id":21204,"date":"2017-02-22T21:28:38","date_gmt":"2017-02-22T14:28:38","guid":{"rendered":"http:\/\/tom.ji42.com\/?p=21204"},"modified":"2017-02-22T21:28:38","modified_gmt":"2017-02-22T14:28:38","slug":"ph-buffer-preparation","status":"publish","type":"post","link":"https:\/\/tom.tomwork.net\/?p=21204","title":{"rendered":"pH Buffer Preparation"},"content":{"rendered":"<p><a href=\"https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-21205\" src=\"https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1.jpg\" alt=\"\" width=\"1500\" height=\"872\" srcset=\"https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1.jpg 1500w, https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1-300x174.jpg 300w, https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1-768x446.jpg 768w, https:\/\/tom.tomwork.net\/wp-content\/uploads\/2017\/02\/81F-akxTkpL._SL1500_1-1024x595.jpg 1024w\" sizes=\"auto, (max-width: 1500px) 100vw, 1500px\" \/><\/a><\/p>\n<p><strong>pH 4.01 Buffer Preparation.<\/strong><\/p>\n<p>1. &#8211; Accurately weigh out 8.954 g of Disodium hydrogen phosphate (Na2HPO4.12H2O) and 3.4023 g of potassium dihydrogen phosphate (KH2PO4).<\/p>\n<p>2. &#8211; Dissolve both the above in distilled water and make up to 1L in a 1L volumetric flask. .<\/p>\n<p><!--more-->NOTE: self prepared solutions need to be stored in a fridge to prevent mold growth. However, keep in mind that the pH of your buffer is temperature dependent.<\/p>\n<p><strong>pH 6.86 Buffer Preparation.<\/strong><\/p>\n<p>1. &#8211; Accurately weigh out 10.212 g of Potassium hydrogen phthalate.<\/p>\n<p>2. &#8211; Dissolve in distilled water and make up to 1L in a 1L volumetric flask.<\/p>\n<p>NOTE: self prepared solutions need to be stored in a fridge to prevent mold growth. However, keep in mind that the pH of your buffer is temperature dependent.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>pH 4.01 Buffer Preparation. 1. &#8211; Accurately weigh out 8.954 g of Disodium hydrogen phosphate (Na2HPO4.12H2O) and 3.4023 g of potassium dihydrogen phosphate (KH2PO4). 2. &#8211; Dissolve both the above in distilled water and make up to 1L in a 1L volumetric flask. .<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[188],"tags":[],"class_list":["post-21204","post","type-post","status-publish","format-standard","hentry","category-188"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p6cOVM-5w0","_links":{"self":[{"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/posts\/21204","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21204"}],"version-history":[{"count":1,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/posts\/21204\/revisions"}],"predecessor-version":[{"id":21206,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=\/wp\/v2\/posts\/21204\/revisions\/21206"}],"wp:attachment":[{"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21204"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21204"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tom.tomwork.net\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21204"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}